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干細(xì)胞多能性評(píng)估PCR試劑盒Pluripotency Check
品牌 Code No. 產(chǎn)品名稱 包裝量 價(jià)格（元） 說明書 數(shù)量
Clontech 631965 Pluripotency Check PCR Kit Each ￥9,630
Clontech 631966 Pluripotency Check PCR Primer Set 16 x 25 Rxns ￥5,306
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干細(xì)胞多能性評(píng)估PCR試劑盒
(Stem Cell Pluripotency Status Check Kit with PCR Primers)
The Pluripotency Check PCR Kit用來通過PCR方法監(jiān)控在發(fā)育多能性方面有關(guān)鍵作用的基因，從而判斷小鼠胚胎干細(xì)胞或是iPS是否真實(shí)具有干細(xì)胞多能性（pluripotency）。本產(chǎn)品覆蓋16個(gè)基因，包括9個(gè)在真實(shí)干細(xì)胞中表達(dá)的基因和2個(gè)內(nèi)參基因?？蛻艨梢造`活決定使用哪些基因進(jìn)行實(shí)際檢測(cè)。本產(chǎn)品可以鑒別關(guān)鍵基因的表達(dá)是內(nèi)源性的（endogenous expression）還是外源性的(exogenous expression)。
 
■ Pluripotency Check PCR Primers
Primer set Amplicon size Primer set Amplicon size
Oct3/4 (Total) 485 bp Klf4 739 bp
Oct3/4 (Endogenous) 224 bp Klf4 (Endogenous) 206 bp
Nanog (Total) 364 bp Ecat1 164 bp
Nanog (Endogenous) 223 bp ERas1 210 bp
Sox2 (Total) 193 bp Esg1 376 bp
Sox2 (Endogenous) 297 bp Rex1 287 bp
c-Myc (Total) 228 bp Beta-Actin (Control) 603 bp
c-Myc (Endogenous) 170 bp GAPDH (Control) 568 bp
 
■ 產(chǎn)品特點(diǎn)
Validate the pluripotent status of your stem cell or iPS cell culture using a simple PCR kit
· 16 prevalidated PCR primer mixes
· Complete kit with RNA extraction, reverse transcription, and PCR reagents
· Confirm your iPS cells by differentiating between endogenous and exogenous gene expression
 
■ 產(chǎn)品應(yīng)用
· Validating mouse embryonic stem cell status
· Confirming iPS status
 
Code No. Product Size
631965 Pluripotency Check PCR Kit Each
631966 Pluripotency Check PCR Primer Set 16 × 25 Rxns
 
■ 產(chǎn)品詳情請(qǐng)點(diǎn)擊：
 
Confirming the pluripotent status of an  mES culture. Total RNA was extracted from ES-E14TG2a mouse embryonic stem cells (mES), followed by cDNA synthesis and amplification according to the protocol outlined in PT5086-2. M: 100 bp DNA Marker. Lane1, Total Oct3/4. Lane 2: Endogenous Oct3/4. Lane 3: Total Nanog. Lane 4: Endogenous Nanog. Lane 5: Total c-Myc. Lane 6: Endogenous c-Myc. Lane 7: Total Sox2. Lane 8: Endogenous Sox2. Lane 9: Total Klf4. Lane 10: Ecat1. Lane 11: ERas. Lane 12: Esg1. Lane 13: Rex1. Lane 14: GAPDH (control). Lane 15: Beta-Actin (control). Endogenous Klf4, data not shown.
 

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